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KMID : 1134820110400010089
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 1 p.89 ~ p.93
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Changes in Properties of Deer Antler by Proteolysis and Extraction Conditions
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Kim Jae-Hwa
Yoo Cheol-Jae
Sin Kyung-A
Jang Se-Young
Park Nan-Young
Jeong Yong-Jin
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Abstract
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This study was conducted to investigate the proteolysis and extraction conditions of deer antler for application of food materials. ProteAX (A) was the most effective enzyme for proteolysis of deer antler and the proteolysis condition was 0.5% (w/w) for enzyme concentration and 5 hr for proteolysis time. The effect of mixing enzyme ProteAX (A)＋KFEN 2 (C) treatment in 60℃, 5 hr was investigated; soluble solid and protein content were the highest with A 0.5% (w/w) and B 0.5% (w/w) concentration. Result for DAH (deer antler hydrolysate) and DA (deer antler) prepared with extraction in 95℃ atmospheric pressure (AP, 6～18 hr) and extraction under 120℃ pressure condition (UP, 15～60 min) after hydrolysis on preceding established condition descriptions indicated that difference in pH according to enzyme treatment and extraction conditions was not significant. Sugar content of DA was 1.5oBrix, DA-UP (under pressure) and DAH-AP (atmospheric pressure) were 2.2°Brix; the highest sugar content of 2.7°Brix was observed in DAH-UP for 60 min extraction. Also total free sugar, crude protein and collagen content were the highest in DAH-UP for 60 min recording at 1.97%, 742.7 ㎎/100 g and 498.8 ㎎/100 g, respectively. From these results, deer antler hydrolysate prepared with extraction under pressure was the most effective for functional characteristics enhancement. Hereafter, various practical uses of materials with enhanced characteristics of antler is expected.
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KEYWORD
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deer antler, proteolysis, proteolytic enzyme, collagen, extraction
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